Rapid transcriptional autoregulation of a yeast metalloregulatory transcription factor is essential for high-level copper detoxification.
Identifieur interne : 002C03 ( Main/Exploration ); précédent : 002C02; suivant : 002C04Rapid transcriptional autoregulation of a yeast metalloregulatory transcription factor is essential for high-level copper detoxification.
Auteurs : P. Zhou ; D J ThieleSource :
- Genes & development [ 0890-9369 ] ; 1993.
Descripteurs français
- KwdFr :
- ADN recombiné (MeSH), ARN messager (métabolisme), Candida (génétique), Candida (métabolisme), Cinétique (MeSH), Cuivre (métabolisme), Données de séquences moléculaires (MeSH), Facteurs de transcription (génétique), Facteurs de transcription (métabolisme), Homéostasie (MeSH), Métallothionéine (génétique), Métallothionéine (métabolisme), Protéines de liaison à l'ADN (génétique), Protéines de liaison à l'ADN (métabolisme), Protéines fongiques (MeSH), Régions promotrices (génétique) (MeSH), Régulation de l'expression des gènes fongiques (MeSH), Sites de fixation (MeSH), Séquence nucléotidique (MeSH), Transcription génétique (MeSH).
- MESH :
- génétique : Candida, Facteurs de transcription, Métallothionéine, Protéines de liaison à l'ADN.
- métabolisme : ARN messager, Candida, Cuivre, Facteurs de transcription, Métallothionéine, Protéines de liaison à l'ADN.
- ADN recombiné, Cinétique, Données de séquences moléculaires, Homéostasie, Protéines fongiques, Régions promotrices (génétique), Régulation de l'expression des gènes fongiques, Sites de fixation, Séquence nucléotidique, Transcription génétique.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Binding Sites (MeSH), Candida (genetics), Candida (metabolism), Copper (metabolism), DNA, Recombinant (MeSH), DNA-Binding Proteins (genetics), DNA-Binding Proteins (metabolism), Fungal Proteins (MeSH), Gene Expression Regulation, Fungal (MeSH), Homeostasis (MeSH), Kinetics (MeSH), Metallothionein (genetics), Metallothionein (metabolism), Molecular Sequence Data (MeSH), Promoter Regions, Genetic (MeSH), RNA, Messenger (metabolism), Transcription Factors (genetics), Transcription Factors (metabolism), Transcription, Genetic (MeSH).
- MESH :
- chemical , genetics : DNA-Binding Proteins, Metallothionein, Transcription Factors.
- chemical , metabolism : Copper, DNA-Binding Proteins, Metallothionein, RNA, Messenger, Transcription Factors.
- genetics : Candida.
- metabolism : Candida.
- Base Sequence, Binding Sites, DNA, Recombinant, Fungal Proteins, Gene Expression Regulation, Fungal, Homeostasis, Kinetics, Molecular Sequence Data, Promoter Regions, Genetic, Transcription, Genetic.
Abstract
Copper detoxification in the yeast Candida glabrata is carried out in large part by a family of metallothionein (MT) genes: a unique MT-I gene, a tandemly amplified MT-IIa gene, and a single unlinked MT-IIb gene. In response to elevated environmental copper levels, members of this MT gene family are transcriptionally activated by a copper-dependent, sequence-specific DNA-binding transcription factor, AMT1. AMT1 shares several structural and functional features with the Saccharomyces cerevisiae copper metalloregulatory transcription factor ACE1, which is constitutively expressed and poised for rapid transcriptional responses to the toxic metal copper. In this paper, we demonstrate that AMT1 is subject to positive transcriptional autoregulation, which is exerted through binding of copper-activated AMT1 to a single copper responsive element in the AMT1 promoter. A nonautoregulatory amt1 mutant displayed a marked decrease in both copper tolerance and expression of the MT-II genes, which are critical for high-level copper detoxification in Candida glabrata. Kinetic analysis demonstrated the remarkably rapid AMT1 mRNA accumulation in the presence of copper, which is followed by increased expression of the metallothionein gene products. These results demonstrate that AMT1-positive autoregulation plays a critical role in metal detoxification and suggest that the rapid autoactivation of the AMT1 metalloregulatory transcription factor biosynthesis is essential for C. glabrata to quickly build up a cellular defense line to protect cells upon exposure to high environmental copper levels.
DOI: 10.1101/gad.7.9.1824
PubMed: 8370529
Affiliations:
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Zhou</name><affiliation><nlm:affiliation>Department of Biological Chemistry, University of Michigan School, Ann Arbor 48109-0606.</nlm:affiliation><wicri:noCountry code="subField">Ann Arbor 48109-0606</wicri:noCountry></affiliation></author><author><name sortKey="Thiele, D J" sort="Thiele, D J" uniqKey="Thiele D" first="D J" last="Thiele">D J Thiele</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1993">1993</date><idno type="RBID">pubmed:8370529</idno><idno type="pmid">8370529</idno><idno type="doi">10.1101/gad.7.9.1824</idno><idno type="wicri:Area/Main/Corpus">002C01</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002C01</idno><idno type="wicri:Area/Main/Curation">002C01</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002C01</idno><idno type="wicri:Area/Main/Exploration">002C01</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Rapid transcriptional autoregulation of a yeast metalloregulatory transcription factor is essential for high-level copper detoxification.</title><author><name sortKey="Zhou, P" sort="Zhou, P" uniqKey="Zhou P" first="P" last="Zhou">P. Zhou</name><affiliation><nlm:affiliation>Department of Biological Chemistry, University of Michigan School, Ann Arbor 48109-0606.</nlm:affiliation><wicri:noCountry code="subField">Ann Arbor 48109-0606</wicri:noCountry></affiliation></author><author><name sortKey="Thiele, D J" sort="Thiele, D J" uniqKey="Thiele D" first="D J" last="Thiele">D J Thiele</name></author></analytic><series><title level="j">Genes & development</title><idno type="ISSN">0890-9369</idno><imprint><date when="1993" type="published">1993</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Binding Sites (MeSH)</term><term>Candida (genetics)</term><term>Candida (metabolism)</term><term>Copper (metabolism)</term><term>DNA, Recombinant (MeSH)</term><term>DNA-Binding Proteins (genetics)</term><term>DNA-Binding Proteins (metabolism)</term><term>Fungal Proteins (MeSH)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Homeostasis (MeSH)</term><term>Kinetics (MeSH)</term><term>Metallothionein (genetics)</term><term>Metallothionein (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Promoter Regions, Genetic (MeSH)</term><term>RNA, Messenger (metabolism)</term><term>Transcription Factors (genetics)</term><term>Transcription Factors (metabolism)</term><term>Transcription, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN recombiné (MeSH)</term><term>ARN messager (métabolisme)</term><term>Candida (génétique)</term><term>Candida (métabolisme)</term><term>Cinétique (MeSH)</term><term>Cuivre (métabolisme)</term><term>Données de séquences moléculaires (MeSH)</term><term>Facteurs de transcription (génétique)</term><term>Facteurs de transcription (métabolisme)</term><term>Homéostasie (MeSH)</term><term>Métallothionéine (génétique)</term><term>Métallothionéine (métabolisme)</term><term>Protéines de liaison à l'ADN (génétique)</term><term>Protéines de liaison à l'ADN (métabolisme)</term><term>Protéines fongiques (MeSH)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Sites de fixation (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Transcription génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA-Binding Proteins</term><term>Metallothionein</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Copper</term><term>DNA-Binding Proteins</term><term>Metallothionein</term><term>RNA, Messenger</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Candida</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Candida</term><term>Facteurs de transcription</term><term>Métallothionéine</term><term>Protéines de liaison à l'ADN</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Candida</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Candida</term><term>Cuivre</term><term>Facteurs de transcription</term><term>Métallothionéine</term><term>Protéines de liaison à l'ADN</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Binding Sites</term><term>DNA, Recombinant</term><term>Fungal Proteins</term><term>Gene Expression Regulation, Fungal</term><term>Homeostasis</term><term>Kinetics</term><term>Molecular Sequence Data</term><term>Promoter Regions, Genetic</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN recombiné</term><term>Cinétique</term><term>Données de séquences moléculaires</term><term>Homéostasie</term><term>Protéines fongiques</term><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes fongiques</term><term>Sites de fixation</term><term>Séquence nucléotidique</term><term>Transcription génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Copper detoxification in the yeast Candida glabrata is carried out in large part by a family of metallothionein (MT) genes: a unique MT-I gene, a tandemly amplified MT-IIa gene, and a single unlinked MT-IIb gene. In response to elevated environmental copper levels, members of this MT gene family are transcriptionally activated by a copper-dependent, sequence-specific DNA-binding transcription factor, AMT1. AMT1 shares several structural and functional features with the Saccharomyces cerevisiae copper metalloregulatory transcription factor ACE1, which is constitutively expressed and poised for rapid transcriptional responses to the toxic metal copper. In this paper, we demonstrate that AMT1 is subject to positive transcriptional autoregulation, which is exerted through binding of copper-activated AMT1 to a single copper responsive element in the AMT1 promoter. A nonautoregulatory amt1 mutant displayed a marked decrease in both copper tolerance and expression of the MT-II genes, which are critical for high-level copper detoxification in Candida glabrata. Kinetic analysis demonstrated the remarkably rapid AMT1 mRNA accumulation in the presence of copper, which is followed by increased expression of the metallothionein gene products. These results demonstrate that AMT1-positive autoregulation plays a critical role in metal detoxification and suggest that the rapid autoactivation of the AMT1 metalloregulatory transcription factor biosynthesis is essential for C. glabrata to quickly build up a cellular defense line to protect cells upon exposure to high environmental copper levels.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8370529</PMID><DateCompleted><Year>1993</Year><Month>10</Month><Day>13</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>16</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0890-9369</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>9</Issue><PubDate><Year>1993</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Genes & development</Title><ISOAbbreviation>Genes Dev</ISOAbbreviation></Journal><ArticleTitle>Rapid transcriptional autoregulation of a yeast metalloregulatory transcription factor is essential for high-level copper detoxification.</ArticleTitle><Pagination><MedlinePgn>1824-35</MedlinePgn></Pagination><Abstract><AbstractText>Copper detoxification in the yeast Candida glabrata is carried out in large part by a family of metallothionein (MT) genes: a unique MT-I gene, a tandemly amplified MT-IIa gene, and a single unlinked MT-IIb gene. In response to elevated environmental copper levels, members of this MT gene family are transcriptionally activated by a copper-dependent, sequence-specific DNA-binding transcription factor, AMT1. AMT1 shares several structural and functional features with the Saccharomyces cerevisiae copper metalloregulatory transcription factor ACE1, which is constitutively expressed and poised for rapid transcriptional responses to the toxic metal copper. In this paper, we demonstrate that AMT1 is subject to positive transcriptional autoregulation, which is exerted through binding of copper-activated AMT1 to a single copper responsive element in the AMT1 promoter. A nonautoregulatory amt1 mutant displayed a marked decrease in both copper tolerance and expression of the MT-II genes, which are critical for high-level copper detoxification in Candida glabrata. Kinetic analysis demonstrated the remarkably rapid AMT1 mRNA accumulation in the presence of copper, which is followed by increased expression of the metallothionein gene products. These results demonstrate that AMT1-positive autoregulation plays a critical role in metal detoxification and suggest that the rapid autoactivation of the AMT1 metalloregulatory transcription factor biosynthesis is essential for C. glabrata to quickly build up a cellular defense line to protect cells upon exposure to high environmental copper levels.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>P</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Biological Chemistry, University of Michigan School, Ann Arbor 48109-0606.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thiele</LastName><ForeName>D J</ForeName><Initials>DJ</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>GM41840</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>M01-RR00042</GrantID><Acronym>RR</Acronym><Agency>NCRR NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Genes Dev</MedlineTA><NlmUniqueID>8711660</NlmUniqueID><ISSNLinking>0890-9369</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004274">DNA, Recombinant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004268">DNA-Binding Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>141490-28-0</RegistryNumber><NameOfSubstance UI="C069807">AMT1 protein, Candida glabrate</NameOfSubstance></Chemical><Chemical><RegistryNumber>789U1901C5</RegistryNumber><NameOfSubstance UI="D003300">Copper</NameOfSubstance></Chemical><Chemical><RegistryNumber>9038-94-2</RegistryNumber><NameOfSubstance UI="D008668">Metallothionein</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><GeneSymbolList><GeneSymbol>AMT1</GeneSymbol><GeneSymbol>CUP1</GeneSymbol><GeneSymbol>MT</GeneSymbol><GeneSymbol>MT-II</GeneSymbol></GeneSymbolList><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base 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Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="Y">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006706" MajorTopicYN="N">Homeostasis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008668" MajorTopicYN="N">Metallothionein</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="Y">Transcription, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1993</Year><Month>9</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1993</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1993</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8370529</ArticleId><ArticleId IdType="doi">10.1101/gad.7.9.1824</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Thiele, D J" sort="Thiele, D J" uniqKey="Thiele D" first="D J" last="Thiele">D J Thiele</name><name sortKey="Zhou, P" sort="Zhou, P" uniqKey="Zhou P" first="P" last="Zhou">P. 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